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Many road signs warned Tommy of the construction work ahead, he did not realize that his lane was closed, though, until he mowed down a traffic barricade with the front of his car.
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Many road signs warned Tommy of the construction work ahead. He did not realize that his lane was closed, though, until he mowed down a traffic barricade with the front of his car.

Many road signs warned Tommy of the construction work ahead is a main clause. He did not realize is a second main clause.

You are always correct when you use a period between the two main clauses that create the comma splice.

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